Heat acclimation and the role of RpoS in prolonged heat shock of Escherichia coli O157

► 39 Proteins underwent induction upon heat stress or rpoS mutation. ► The heat post-acclimation stimulon consists of stress and housekeeping proteins. ► RpoS has limited control in protein translation, elongation and folding. ► RpoS showed significant control over cellular metabolic processes.