Multiplex PCR assay for the detection of aflatoxigenic and non-aflatoxigenic fungi in meju, a Korean fermented soybean food starter

► We developed multiplex PCR to detect aflatoxigenic fungi in meju. ► Two different sets of three primers were designed for the omtB, ver-1, aflR, and omtA genes. ► Only aflatoxigenic Aspergillus species gave complete amplification in both primer sets. ► This method may provide an accurate and specific detection of aflatoxigenic Aspergillus in meju.