Evaluation of shiga toxin-producing Escherichia coli (STEC) method for the detection and identification of STEC O104 strains from sprouts

► Detection and recovery of STEC, including Escherichia coli O104, from sprouts. ► Real-time PCR assays evaluated for detection of STEC, specifically E. coli O104. ► Evaluation of enrichment and isolation media for recovery of non-O157 STEC strains.