Ontogeny of osmoregulatory patterns in the South American shrimp Macrobrachium amazonicum: Loss of hypo-regulation in a land-locked population indicates phylogenetic separation from estuarine ancestors

►Osmoregulation is a key adaptation for limnic invasions. ►Different patterns of osmoregulation reflect different life styles. ►Ontogenetic changes in osmoregulation reflect different life-history strategies. ►A hololimnetic clade of shrimp has lost the capability of hypo-osmoregulation. ►Diadromous and hololimnetic shrimp populations show signs of phylogenetic separation.