Quantitative proteomic analysis reveals the mode-of-action for chronic mercury hepatotoxicity to marine medaka (Oryzias melastigma)

► Mercury (Hg) is a widespread persistent pollutant in aquatic ecosystems. ► The precise mechanism of chronic Hg hepatotoxicity in organisms is not well known. ► A proteomic study of medaka liver by Hg has novel and significant characteristics. ► We identified 33 differential protein spots that associate with Hg hepatotoxicity. ► The proteomic analysis elucidates the mode-of-action of chronic Hg hepatotoxicity.