Cytochrome P450 1C1 complementary DNA cloning, sequence analysis and constitutive expression induced by benzo-a-pyrene in Nile tilapia (Oreochromis niloticus)

► We cloned a new c DNA of the CYP1C subfamily encoding CYP1C1 Nile tilapia liver after intraperitoneal injection with benzo-a-pyrene (BaP). ► The full-length cDNA was 2223 bp long and contained an open reading frame of 1581 bp. ► The phylogenetic tree shows tilapia CYP1C1 and scup CYP1C1 to be more closely related to each other. ► We measured benzo-a-pyrene (BaP) induction of CYP1C1 mRNA in different organs of tilapia. ► Real time RCR results revealed that there was a large increase in CYP1C1 mRNA in liver (43.1), intestine (5.1) and muscles (2.4).