Consumption of benthic cyanobacterial mats and nodularin-R accumulation in freshwater crayfish (Paranephrops planifrons) in Lake Tikitapu (Rotorua, New Zealand)

► Isotopically labeled 13C feeding studies confirmed benthic mats are consumed by kōura. ► Nodularin-R was detected in kōura for the first time. ► This is this first detection of nodularin-R in a freshwater organsims in a freshwater system. ► Demonstrates accumulation of nodularin-R from freshwater benthic cyanobacterial mats. ► Benthic mats need to be considered in future cyanotoxins freshwater food-web studies.