کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6389380 1628220 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Occurrence and characterization of a tetrahedral nucleopolyhedrovirus from Spilarctia obliqua (Walker)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Occurrence and characterization of a tetrahedral nucleopolyhedrovirus from Spilarctia obliqua (Walker)
چکیده انگلیسی


- Spilarctia obliqua is a polyphagous pest of many crops in the oriental region.
- We have isolated a new tetrahedral shaped nucleopolyhedrovirus infecting this pest.
- The virus was characterized and evaluated for its virulence against the pest.
- Isolation of a virulent virus holds promise to develop it as a bio-pesticide.

Spilarctia obliqua Walker (Lepidoptera: Arctiidae) is a polyphagous insect pest damaging pulses, oil seeds, cereals, vegetables and medicinal and aromatic plants in India. The pest also infests turmeric and ginger sporadically in Kerala. We observed an epizootic caused by a nucleopolyhedrovirus (NPV) in field populations of the insects in December 2013. The NPV was purified and characterized. The isolate was tetrahedral in shape and belonged to multicapsid NPV. The REN profile of the SpobNPV genome with Pst I, Xho I and HindIII enzymes showed a genome size of 99.1 ± 3.9 kbp. Partialpolh, lef-8 and lef-9 gene sequences of the isolate showed a close relationship with HycuNPV and SpphNPV. Phylogram and K-2-P distances between similar isolates suggested inclusion of the present SpobNPV isolate to group I NPV. The biological activity of the isolate was tested under laboratory conditions against third instar larvae of S. obliqua and the LC50 was 4.37 × 103 OBs/ml occlusion bodies (OBs) per ml. The median survival time (ST50) was 181 h at a dose of 1 × 106 OBs/ml and 167 h at a dose of 1 × 108 OBs/ml. SpobNPV merits further field evaluation as a potential biological control agent of S. obliqua, a serious pest of many agriculturally important crops in the Oriental region.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Invertebrate Pathology - Volume 132, November 2015, Pages 135-141
نویسندگان
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