Intercommunity effects on microbiome and GpSGHV density regulation in tsetse flies

► Three symbionts and a DNA virus reside in different tsetse tissues. ► Availability of GmmWig− and GmmApo enables analysis of intercommunity dependencies. ► The symbiont densities are tightly regulated through host development. ► There is cooperative interaction between Wigglesworthia and Sodalis. ► DNA virus is commensal without displaying pathogenesis in the absence of symbionts.