A multi-gene phylogeny provides additional insight into the relationships between several Ascosphaera species

► Ascosphaera fungi cause chalkbrood disease in several bee species. ► We use up to six loci to generate phylogenies for Ascosphaera species. ► Using all six loci results in the best supported phylogeny. ► Pathogenic and apathogenic Ascosphaera cluster together in our phylogenies. ► With further study, Ascosphaera may be paraphylectic.