کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6389813 1329917 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short CommunicationProfiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Short CommunicationProfiling of a few immune responsive genes expressed in postlarvae of Fenneropenaeus indicus challenged with Vibrio harveyi D3
چکیده انگلیسی

We identified 38 genes and eight hypothetical proteins by sequencing of 1200 clones from a Vibrio harveyi challenged Fenneropenaeus indicus subtracted cDNA library. Based on physiological roles and functions these genes were categorized into 10 groups with ∼29% of the sequences having no matches in the databases. Immune related transcripts in the library were carboxypeptidase B, ferritin, translationally controlled tumor proteins (TCTP), hemocyanin, chitinase and serine carboxy peptidase. Remarkably, qPCR results imply 4.15, 3.45 and 1.86-fold rises in expression of ferritin, TCTP and hemocyanin transcripts respectively. Additionally, minor upregulation of other immune relevant genes lectin, penaeidin, crustin, MnSOD was observed in the challenged postlarvae.

qPCR expression profile of different immune related genes in Fenneropenaeus indicus after the Vibrio harveyi challenge. .Highlights► In this study, using SSH we identified 38 genes and eight hypothetical proteins in V. harveyi challenged F. indicus postlarvae. ► Real time gene expression analyses showed rise in expression levels of all seven different immune related transcripts in the postlarvae. ► Ferritn, translationally controlled tumor protein and hemocyanin were the major upregulated genes in V. harveyi challenged postlarvae.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Invertebrate Pathology - Volume 107, Issue 2, June 2011, Pages 168-172
نویسندگان
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