Microbial oils as food additives: recent approaches for improving microbial oil production and its polyunsaturated fatty acid content

- Oleaginous fungi and microalgae are suitable sources of PUFAs.
- Genetic engineering for improving oil accumulation and fatty acid composition.
- Overexpression of key-enzymes involved in lipid biosynthesis.
- Repression of β-oxidation pathway.
- Enhancement of PUFA biosynthesis via genetic manipulation.