Building cellular pathways and programs enabled by the genetic diversity of allo-genomes and meta-genomes

- Rational design and selection-based cell engineering can both be applied for development of complex traits and pathways.
- Both genetic diversity and enrichment methods play critical roles in selected genetic determinants.
- The metagenome contains a diverse genomic space and can be screened for desirable traits.
- Growth and assay based technologies including biosensors provide the framework for high throughput screening tools.
- A modular, iterative engineering approach can be applied to develop complex pathways and traits.