Application of isotope labeling experiments and 13C flux analysis to enable rational pathway engineering

- ILEs can guide pathway engineering to improve product titer, rate, or yield.
- ILEs have been used to characterize the metabolism of non-model organisms.
- ILEs have been used to identify wasteful processes that lead to yield losses.
- ILEs have been used to identify kinetic bottlenecks in metabolic networks.
- Industry can benefit from the utilization of ILEs to optimize bioprocesses.