Novel screening methods - biosensors

- Transcription factors are approved sensors to monitor intracellular concentrations.
- Sensors coupled to gfp expression convert concentrations into a graded signal.
- Combination of FACS and sensors enable selection of target-molecule-producing microorganisms.
- NAD(P)H-responsive sensors offer generalizable screens for NAD(P)H-dependent enzymes.
- Recombineering plus sensors unite diversity generation and producer selection.