How to measure metabolic fluxes: a taxonomic guide for 13C fluxomics

- Tracer experiments, analytics and modeling constitute the 13C fluxomics triumvirate.
- 13C fluxomics tools differ in complexity, information power and universality.
- The biological question prescribes the choice of the modeling approach.
- In turn, modeling poses unique challenges for experimental and analytical setups.
- Standards will raise throughput and flexibility of 13C fluxomics.