A roadmap for interpreting 13C metabolite labeling patterns from cells

- 13C tracer analysis can be used to probe intracellular metabolism in cells.
- Steady state labeling patterns can quantify nutrient contributions.
- Steady state labeling patterns can inform about pathway activities.
- Dynamic labeling patterns combined with metabolite levels can quantify some fluxes.
- Analysis of multiple metabolites and various tracers enhances information output.