High throughput screening of enzyme activity with mass spectrometry imaging

- Mass spectrometry is a versatile alternative to colorimetric enzyme activity assays.
- Technologies developed for MSI can be applied to high-throughput enzyme screening.
- Analyte immobilization allows for activity determination in complex sample matrices.
- Such methods along with selective derivatization reactions enable label-free assays.
- These advances could aid the spatial localization of enzymatic activity with MSI.