Riboswitch engineering - making the all-important second and third steps

- Addition of in vivo active aptamer domains for DNT and PPDA to the toolbox.
- Addition of expression platforms for transcriptional termination to the toolbox.
- Modular 'plug-and-play' combination of aptamer domains and expression platforms.
- Combined in vivo screens and structure analysis will improve riboswitch regulation.
- Riboswitches are functional in all kingdoms of life, as well as in viruses.