Secondary ion mass spectrometry and Raman spectroscopy for tissue engineering applications

- TOF-SIMS and Raman spectroscopy are label-free chemical imaging techniques.
- Biomaterial surface chemistry can be imaged and identified with TOF-SIMS.
- MVA of TOF-SIMS data can correlate surface chemistry with cell response.
- Raman spectroscopy can identify the differentiation stages of single, living cells.
- The stage of cell cycle can be determined with Raman spectroscopy.