Measurement and control of host cell proteins (HCPs) in CHO cell bioprocesses

- Cell viability and time of harvest impacts greatly on the subsequent HCP profile.
- Primary and secondary clarification decisions impact on the HCP profile.
- HCPs in protein A chromatography eluates appear to predominately depend on HCP-product interactions due to co-elution of HCPs with the product.
- The degree of resin-HCP interaction depends on the matrix utilised.
- Different subsets of HCPs may interact with different mAbs.