Mammalian cell-produced therapeutic proteins: heterogeneity derived from protein degradation

- Mammalian cell-produced therapeutic glycoproteins are heterogeneous in nature.
- Causes of product heterogeneity are highlighted.
- Examples of protein degradation by non-enzymatic and enzymatic pathways are reviewed.
- Strategies used for controlling protein degradation are described.
- Possible development of host cell lines with minimal degradative properties is discussed.