Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates

- Access to cellulose by cellulase limits the efficiency of enzymatic hydrolysis.
- Access to cellulose is mainly through the pores in the cell wall rather than external surface area.
- Different pretreatment effects cellulose accessibility to different extent through different mechanisms.
- Lignin and hemicellulose plays important role in effecting cellulose accessibility.
- Accurate assess cellulose accessibility needs multiple analytical techniques.