Application of a new model based on oxygen balance to determine the oxygen uptake rate in mammalian cell chemostat cultures

The proposed model was compared with a model that only includes the OUR term (typical model). The standard error of the proposed model was less than that of the typical model, making the proposed model more accurate. The results showed that at 37 °C, the results significantly differed depending on which model was used. At 33 and 37 °C, the specific oxygen uptake rate (qO2) values obtained with the proposed model were within the range usually reported for mammalian cells. It was also shown that the OUR value was underestimated if the oxygen transfer through the headspace-medium interface was not considered under mild hypothermia conditions. The model could be applied for developing strategies based on OUR monitoring and controlling for recombinant protein production under conditions of mild hypothermia.