Model-based characterization of operational stability of multimeric enzymes with complex deactivation behavior: An in-silico investigation

► Multimeric enzymes require multimeric deactivation models for stability characterization. ► The often used Lumry-Eyring deactivation models is insufficient as empiric description for multimer deactivation. ► Conversion in a continuous enzyme membrane reactor allows model discrimination and yields reliable characterizations. ► Relaxed stochastic criteria allow for identification of parameterization applicable to process design.