کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6809400 | 1433596 | 2012 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Different β-amyloid oligomer assemblies in Alzheimer brains correlate with age of disease onset and impaired cholinergic activity
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کلمات کلیدی
β-amyloid oligomers - الیگومرهای β-آمیلوئیدAlzheimer's disease - بیماری آلزایمرEarly-onset Alzheimer's disease - بیماری آلزایمر در اوایل شروعLate-onset Alzheimer's disease - بیماری آلزایمر در شروع زودرسAge - سنCholine acetyltransferase activity - فعالیت کولین استیل ترانسفرازnicotinic acetylcholine receptors - گیرنده های استیل کولین نیکوتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, we examined the relationship between various β-amyloid (Aβ) oligomer assemblies in autopsy brain with the levels of fibrillar Aβ and cholinergic synaptic function. Brain tissues obtained from the frontal cortex of 14 Alzheimer's disease (AD) patients grouped into early-onset AD (EOAD) and late-onset AD (LOAD) and 12 age-matched control subjects were used to extract and quantify Aβ oligomers in soluble (TBS), detergent soluble (TBST), and insoluble (GuHCl) fractions. The predominant oligomeric Aβ assemblies detected were dodecamers, decamers, and pentamers, and different patterns of expression were observed between EOAD and LOAD patients. There was no association between any of the detected Aβ oligomer assemblies and fibrillar Aβ levels measured by N-methyl[3H] 2-(40-methylaminophenyl)-6-hydroxy-benzothiazole ([3H]PIB) binding. Levels of pentamers in the soluble fraction significantly correlated with a reduction in choline acetyltransferase activity in AD patients. The number of nicotinic acetylcholine receptors negatively correlated with the total amount of Aβ oligomers in the insoluble fraction in EOAD patients, and with decamers in the soluble fraction in LOAD patients. These novel findings suggest that distinct Aβ oligomers induce impairment of cholinergic neurotransmission in AD pathogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Aging - Volume 33, Issue 4, April 2012, Pages 825.e1-825.e13
Journal: Neurobiology of Aging - Volume 33, Issue 4, April 2012, Pages 825.e1-825.e13
نویسندگان
Fuxiang Bao, Linn Wicklund, Pascale N. Lacor, William L. Klein, Agneta Nordberg, Amelia Marutle,