Detection of Sphingomonas paucimobilis and antibacterial activity of Prosopis farcta extracts on it

Six isolates of Sphingomonas paucimobilis were isolated from 120 hospital workers hands in Erbil city/Iraq by using VITEK2 Compact system, then further confirmed by PCR technique and by detecting specific gene TDP-glucose pyrophosphorylase (320bp) for S. paucimobilis ATCC 31461 and all local isolates. The effect of 17 antibiotics against isolated bacteria was studied and the results showed that all isolates were 100% sensitive to Piperacillin/Tazobactam, Imipenem, Meropenem, Ciprofloxacin, and Levofloxacin, while it was 83.33% resistant to Cefoxitin and Cefepime. The minimum inhibitory concentration (MIC) of Prosopis farcta pods extracts against S. paucimobilis isolate (S.p4) was 1000 μg/ml for methanol and ethanol extracts and 1200 μg/ml of watery extract. Moreover, the inhibitory effect of the above plant extracts (sub MIC) was studied against bacterial plasmid and the results showed three plasmid DNA bands when treated with 1000 μg/ml for watery extract and missing one band after treating with 800 μg/ml for both methanol and ethanol extracts. Also, the results of inhibitory effects of mentioned plant extracts on total protein by using SDS-PAGE showed the differences in protein banding pattern which 32 bands was formed when treated with 1000 μg/ml for watery extract and it was decreased to 16 bands when treated with 800 μg/ml for both methanol and ethanol extract.