Protein binding pigment by Bacillus pumilus SF214

In order to prepare spores of Bacillus pumilus SF214, sporulation was induced by exhaustion method. Electrophoresis of proteins on polyacrylamide gels (SDS-PAGE) was done using extracted of proteins coating spores, for an aliquot of spores treated with 0.1 M NaOH and to determine the sequence of protein band we used Edman degradation technique and the database (BLASTp) software. Protein extracts of red and white spores showed several differences for what concerns the intensity of some protein bands. Clear increasing in pigment production with the progression of the stationary phase of growth. The analysis of protein band transferred on nitrocellulose membrane using Edman degradation technique showed that the sequence alignment obtained is the homologous N-terminal protein of Bacillus subtilis Tasa. This study suggests that pigmentation in spores represents an additional and maybe alternative protection strategy against oxidative stress. For the first time, this study was performed to describe the correlation between the pigment, protein and spore formation, Which support the proposed application in the field of food or medical production as probiotics, this study was performed to describe the correlation between the pigment, protein and spore formation, Which support the proposed application in the field of food or medical production as probiotics.