An adaptive positivity thresholding method for automated Ki67 hotspot detection (AKHoD) in breast cancer biopsies

The method has been preliminarily validated on 50 digital slides for which three expert pathologists provided gold standard hotspots. 82% of the gold standard hotspots have been successfully recognized by the system, spending an average of 54 s per slide. While further validation is needed taking into account also patients follow-up, this first experimentation suggests that the proposed method could be adequate for supporting the pathologist in hotspot detection.