کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6921101 | 864443 | 2015 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel multi-scale Hessian based spot enhancement filter for two dimensional gel electrophoresis images
ترجمه فارسی عنوان
یک فیلتر جدید تقویت نقطه ای مبتنی بر حساسیت چند بعدی برای تصاویر الکتروفورز ژل دو بعدی
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کلمات کلیدی
فیلتر افزایش تصحیح، الکتروفورز ژل دو بعدی، تشخیص تقسیم بندی، پروتئومیکس، هسیان،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی کامپیوتر
نرم افزارهای علوم کامپیوتر
چکیده انگلیسی
Two dimensional gel electrophoresis (2DGE) is a useful method for studying proteins in a wide variety of applications including identifying post-translation modification (PTM), biomarker discovery, and protein purification. Computerized segmentation and detection of the proteins are the two main processes that are carried out on the scanned image of the gel. Due to the complexities of 2DGE images and the presence of artifacts, the segmentation and detection of protein spots in these images are non-trivial, and involve supervised and time consuming processes. This paper introduces a new spot filter for enhancing, and separating the closely overlapping spots of protein in 2DGE images based on the multi-scale eigenvalue analysis of the image Hessian. Using a Gaussian spot model, we have derived closed form equations to compute the eigen components of the image Hessian of two overlapping spots in a multi-scale fashion. Based on this analysis, we have proposed a novel filter that suppresses the overlapping area and results in a better spot separation. The performance of the proposed filter has been evaluated on the synthetic and real 2DGE images. The comparison with three conventional techniques and a commercial software package reveals the superiority and effectiveness of the proposed filter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Computers in Biology and Medicine - Volume 66, 1 November 2015, Pages 154-169
Journal: Computers in Biology and Medicine - Volume 66, 1 November 2015, Pages 154-169
نویسندگان
Sina Shamekhi, Mohammad Hossein Miran Baygi, Bahareh Azarian, Ali Gooya,