کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7063141 | 1459799 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of polyhydroxybuyrate (PHB) from switchgrass pretreated with a radio frequency-assisted heating process
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Effects of radio frequency (RF) heating as a biomass pretreatment process to generate hydrolysates for polyhydroxybuyrate (PHB) were evaluated in all production steps from pretreatment to enzymatic hydrolysis to fermentation. Switchgrass was pretreated under alkaline conditions with RF-assisted heating (traditional water bath (WB) heating as control) and subsequently enzymatically hydrolyzed. Fermentation was conducted with recombinant Escherichia coli strain for PHB production using the hydrolysates as carbon sources with or without yeast extract (YE) supplemented. Results indicated that the hydrolysates generated through RF pretreatment performed consistently better for PHB production than WB (50% higher PHB levels without YE). YE supplementation (up to 5Â g/L) enhanced fermentation under all conditions and diminished the difference among performances of fermentations. When adding 2Â g/L YE, PHB production increased by 200% and 80% for WB and RF hydrolysates, respectively. Supplementation of 5Â g/L YE brought the final PHB concentration to be very close to each other for all three fermentation conditions. Compared to traditional heating process, the unique heating mechanism of RF generates harsher conditions (under regular pressure) to disrupt the biomass structure more completely and generate more nutrients for bacterial fermentation. RF was therefore proved to be an efficient process for biomass pretreatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomass and Bioenergy - Volume 94, November 2016, Pages 220-227
Journal: Biomass and Bioenergy - Volume 94, November 2016, Pages 220-227
نویسندگان
Xiaofei Wang, Zhong-Tian Zhang, Yi Wang, Yifen Wang,