کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7114176 | 1461092 | 2015 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Three-dimensional particle tracking in microfluidic channel flow using in and out of focus diffraction
ترجمه فارسی عنوان
ردیابی ذرات سه بعدی در جریان کانال میکروفیلییدی با استفاده از پراش فوکوس و خارج از آن
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
کنترل و سیستم های مهندسی
چکیده انگلیسی
Three-dimensional particle tracking is important to accurately understand the motion of particles within complex flow fields. We show that three-dimensional trajectories of particles within microfluidic flow can be extracted from two-dimensional bright field video microscopy. The method utilizes the defocusing that occurs as particles move out of the objective focal plane when viewed through a high numerical aperture objective lens. A fast and simple algorithm based on cross correlation to a set of reference images taken at prescribed amounts of defocus is used to extract out-of-plane particle position. In-plane particle position is determined through center point detection and therefore the particle position in all three dimensions can be constructed at each time point. Particle trajectories at high flow velocity of greater than 2Â mm/s can be tracked by utilizing a high speed camera to obtain unblurred images. Three dimensional computational fluid simulations are used to validate the particle tracking methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Flow Measurement and Instrumentation - Volume 45, October 2015, Pages 218-224
Journal: Flow Measurement and Instrumentation - Volume 45, October 2015, Pages 218-224
نویسندگان
Bushra Tasadduq, Gonghao Wang, Mohamed El Banani, Wenbin Mao, Wilbur Lam, Alexander Alexeev, Todd Sulchek,