کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7136993 | 1461881 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inkjet monitoring technique with quartz crystal microbalance (QCM) sensor for highly reproducible antibody immobilization
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We demonstrate that a calibrated amount of a biomolecule can be microdeposited using a commercially available quartz crystal microbalance (QCM) and a piezoelectric inkjet head with a flattened surface surrounding the ejection hole. Covering the QCM with a stainless-steel cover perforated with a precisely-machined hole both significantly decreased background noise due to external sources such as air flow and temperature fluctuations and allowed accurate droplet ejection at a constant temperature. Anti-c reactive protein antibody solution (anti-CRP; 1.0Â mg/ml) was continuously ejected onto a microchannel target using a piezoelectric injector; its microdeposition was monitored shot-by-shot using a 30Â MHz QCM. The dispersion of the QCM frequency change per shot decreased from 7.87 to 1.01Â Hz when a perforated stainless-steel cover was installed. The primary antibody was precisely deposited on the target with high reproducibility. The variation in luminescence measured by enzyme-linked immunosorbant assay (ELISA) decreased from 9.67% to 1.16% when calibrating the amount of primary antibody deposition with this developed technique.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators A: Physical - Volume 219, 1 November 2014, Pages 1-5
Journal: Sensors and Actuators A: Physical - Volume 219, 1 November 2014, Pages 1-5
نویسندگان
Y. Fuchiwaki, Y. Yabe, Y. Adachi, M. Tanaka, K. Abe, M. Kataoka, T. Ooie,