کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7138753 | 1462017 | 2018 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Graphene-based enzyme-modified field-effect transistor biosensor for monitoring drug effects in Alzheimer's disease treatment
ترجمه فارسی عنوان
سنسور اثر ترانزیستور اثر میدان مغناطیسی بر اساس گرافن برای کنترل اثر دارو در درمان بیماری آلزایمر
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کلمات کلیدی
کاهش اکسید گرافین، بیماری آلزایمر، بیوسنسور، استیل کولین، مهارکننده استیل کولین استراز،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A reduced graphene oxide-based enzyme-modified field-effect transistor (RGO-EnFET) was fabricated to study the enzymatic kinetics between acetylcholinesterase (AChE) and acetylcholine (ACh), which are related to the progression and treatment of Alzheimer's disease (AD). The RGO-EnFET exhibited typical ambipolar transfer characteristics with a charge neutrality point (the Dirac point, VDirac) in an aqueous environment, and biological events occurring on the RGO surface were evaluated by observing the Dirac point shift (ÎVDirac) according to reactions. First, the pH sensitivity of the RGO-EnFET was measured to be 24.12â¯mVâ¯pHâ1 for a pH range of 4-10 with excellent repeatability. Then, ACh quantification in the concentration range of 1â¯Î¼M-10â¯mM was evaluated, with a linear slope of 13.9â¯mVâ¯decâ1 being obtained between ÎVDirac and the ACh concentration on a logarithmic scale. The effects of AChE inhibitors (donepezil and rivastigmine) on the AChE enzymatic activity were also investigated. The distinguishable inhibition rates were acquired for donepezil and rivastigmine at saturation levels of 90% and 75%, respectively. These results indicate that the RGO-EnFET biosensor was successfully exploited as an in vitro analytical tool and has potential of future application in the study of enzymatic kinetics and drug screening for therapeutic purposes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 272, 1 November 2018, Pages 448-458
Journal: Sensors and Actuators B: Chemical - Volume 272, 1 November 2018, Pages 448-458
نویسندگان
Myung-Sic Chae, Yong Kyoung Yoo, Jinsik Kim, Tae Geun Kim, Kyo Seon Hwang,