Advanced automation strategies for reliable, reproducible cultivation runs in a sequential/parallel operated multi-bioreactor plant

A designed multi-bioreactor plant, comprising a 5 l cell breeding BIOSTAT® Bplus system and a sixfold 1 l BIOSTAT® Qplus system, was equipped with different, interlinked automation structures for systematic process controlling and reliable optimization procedures. Benefits obtained were time saving, assurance and improvement of quality and cost reduction in bioprocess production. In this report the concept for moving beyond the state-of-the-art by creating a fully automated multi-bioreactor plant is explained. The efficiency of the approach is demonstrated by investigation relating the influence of the dissolved oxygen tension pO2 in combination with the methanol concentration cS2M in Malaria vaccine production processes with Pichia pastoris.