کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7139624 | 1462022 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gold nanoparticle triggered dual optoplasmonic-impedimetric sensing of prostate-specific antigen on interdigitated porous silicon platforms
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A porous silicon (PSi) platform with interdigitated NiCr electrodes has been used for the impedimetric biosensing of prostate specific antigen (PSA), a cancer biomarker. The first step involved the formation of a conductive PSi structure through the ion beam creation of 250â¯nm deep interdigitated slots, followed by their filling with NiCr electrodes deposited by plasma sputtering. In the second step, a biorecognition interface was formed by modification of the PSi surface with (3-Glycidyloxypropyl)-trimethoxysilane. A sandwich assay was performed in which PSA was first recognized by a surface-anchored antibody and then by a secondary antibody tethered to 100â¯nm gold nanoparticles (GNPs). The changes in the impedance upon analyte binding at different concentrations have been monitored showing that the PSA concentration exhibits a linear dependence with the series resistance defined from the device equivalent circuit. A limit of detection in the range of 1â¯ng/mL PSA has been determined. A dark-field study of the PSi surface after the sandwich bioassay allowed an optoplasmonic demonstration of the increasing density of GNPs at higher PSA concentrations, which was confirmed by using scanning electron microscopy. The results confirm the dual optoplasmonic-electric labelling effect of the 100â¯nm GNPs and their role in an internally controlled detection of PSA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 267, 15 August 2018, Pages 559-564
Journal: Sensors and Actuators B: Chemical - Volume 267, 15 August 2018, Pages 559-564
نویسندگان
C. Rodriguez, V. Torres-Costa, O. Ahumada, V. Cebrián, C. Gómez-Abad, A. DÃaz, M. Manso Silván,