کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7140234 1462025 2018 26 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Positive carbon dots with dual roles of nanoquencher and reference signal for the ratiometric fluorescence sensing of DNA
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Positive carbon dots with dual roles of nanoquencher and reference signal for the ratiometric fluorescence sensing of DNA
چکیده انگلیسی
Specific modification of carbon dots (CDs) exhibit excellent properties and are suitable for numerous applications. The exploitation of multifunctional CDs in the area of fluorescent assay is still urgent and attractive. Herein, using a simple two-step heating procedure, positive charged carbon dots (P-CDs) were prepared with the co-doping of the elements of N and S. The morphology, optical properties and stability of P-CDs was investigated. Furthermore, P-CDs show the dual functions of unprecedented quenching performance toward fluorophore-modified DNA coupling the retaining fluorescence of P-CDs self. The quenching effect and mechanism of P-CDs to fluorophore-modified DNA was investigated. The fluorescence and quenching properties of P-CDs were used to design a ratiometric fluorescence platform for DNA based on the complex of P-CDs and FAM-labeled ssDNA. The ratiometric method allows the detection of target DNA because of the different affinities of P-CDs to ssDNA and dsDNA with acceptable sensitivity and improved reproducibility, when using sequence fragment of the gene of Gardnerella vaginalis as model. This method provides a new approach to widen the future of the biological applications based on some special CDs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 264, 1 July 2018, Pages 193-201
نویسندگان
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