کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7140416 | 1462028 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel signal amplification system fabricated immunosensor based on Au nanoparticles and mesoporous trimetallic PdPtCu nanospheres for sensitive detection of prostate specific antigen
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this work, the gold nanoparticles (Au NPs) substrate and the prepared mesoporous trimetallic PdPtCu nanospheres (m-PdPtCu) as the label of secondary antibodies (Ab2, Ab2 labeled probe) were used as the basis for fabricating a highly sensitive immunosensor to detect trace amount of prostate specific antigen (PSA) in human serum. The Au NPs can provide a suitable microenvironment for immobilizing primary antibodies (Ab1) and accelerate the electron transfer on the electrode interface due to the good biocompatibility and conductivity. The sensitivity of immunosensor was greatly amplified by the designed novel Ab2 labeled probe, which can be attributed to the m-PdPtCu with mesoporous structure not only can provide high specific surface area, but also offer abundant catalytically active sites and accessible pores for reactants. Under the optimal conditions, a linear relationship between current responses and the logarithm of the PSA concentrations were obtained in the wide range of 10â¯fgâ¯mLâ1-100â¯ngâ¯mLâ1 and the detection limit was 3.3â¯fgâ¯mLâ1. Remarkably, the detection result of PSA in real human serum samples by the immunosensor is well in correlation with commercial ELISA, which confirms that the immunosensor is promising in early clinical detection of biomarkers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 261, 15 May 2018, Pages 22-30
Journal: Sensors and Actuators B: Chemical - Volume 261, 15 May 2018, Pages 22-30
نویسندگان
Mingdang Li, Ping Wang, Fubin Pei, Haoxuan Yu, Yunhui Dong, Yueyun Li, Qing Liu, Peng Chen,