کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7140693 | 1462027 | 2018 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Drug and nanoparticle mediated rapid naked eye water test for pathogens detection
ترجمه فارسی عنوان
دارو و نانوذرات از طریق آزمایش سریع چشم بر روی چشم برای تشخیص پاتوژن انجام شده است
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
کولیستین، تشخیص ویژوال، نانوذرات طلا، تشخیص پاتوژن،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Inspired by the interaction of colistin with lipopolysaccharides (LPS) of the bacterial outer membrane, we describe a simple, cost effective and rapid assay for the detection of bacterial contamination in water samples. Colistin, a bactericidal drug, has been used by us in a receptor configuration for detection of pathogenic microorganisms without involving any tedious sample preparation step. The approach employs the cationic antibiotic drug for dual purpose, firstly, as a primary binder for pathogens and secondly, as an aggregator for negatively charged Gold nanoparticles (GNPs). The former consists of colistin binding to bacteria in water that renders GNPs free in solution thus depicting red color and the latter shows colistin driven aggregation of GNPs producing blue colored solution. The assay works in a two-step procedure that involves addition of colistin and GNPs to the water sample before results can be visualized based on color change. The assay is sensitive at a concentration up to 10 bacterial cells·mLâ1 in a time frame of 5â¯min without requiring any expensive reagents and instruments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 262, 1 June 2018, Pages 603-610
Journal: Sensors and Actuators B: Chemical - Volume 262, 1 June 2018, Pages 603-610
نویسندگان
Pargat Singh, Ritika Gupta, Megha Choudhary, Anil Kumar Pinnaka, Rajesh Kumar, Vijayender Bhalla,