کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7140693 1462027 2018 34 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Drug and nanoparticle mediated rapid naked eye water test for pathogens detection
ترجمه فارسی عنوان
دارو و نانوذرات از طریق آزمایش سریع چشم بر روی چشم برای تشخیص پاتوژن انجام شده است
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Inspired by the interaction of colistin with lipopolysaccharides (LPS) of the bacterial outer membrane, we describe a simple, cost effective and rapid assay for the detection of bacterial contamination in water samples. Colistin, a bactericidal drug, has been used by us in a receptor configuration for detection of pathogenic microorganisms without involving any tedious sample preparation step. The approach employs the cationic antibiotic drug for dual purpose, firstly, as a primary binder for pathogens and secondly, as an aggregator for negatively charged Gold nanoparticles (GNPs). The former consists of colistin binding to bacteria in water that renders GNPs free in solution thus depicting red color and the latter shows colistin driven aggregation of GNPs producing blue colored solution. The assay works in a two-step procedure that involves addition of colistin and GNPs to the water sample before results can be visualized based on color change. The assay is sensitive at a concentration up to 10 bacterial cells·mL−1 in a time frame of 5 min without requiring any expensive reagents and instruments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 262, 1 June 2018, Pages 603-610
نویسندگان
, , , , , ,