کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7140745 | 1462028 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inkjet 3D printed chip for capillary gel electrophoresis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Inkjet 3D printed chip for capillary gel electrophoresis Inkjet 3D printed chip for capillary gel electrophoresis](/preview/png/7140745.png)
چکیده انگلیسی
This paper presents for the first time the use of an inkjet 3D printing to develop a chip for capillary gel electrophoresis. The designing of the chip is preceded by investigations into surface roughness and the geometrical properties of the printed microchannels, which are important considerations for the separation process. The optical properties of the building material are also determined to confirm whether fluorescence detection is possible. It is found that the printing orientation of the microchannels plays a crucial role in the proper operation of the separation process. A description of the successful separation of a 50-800â¯bp DNA ladder, which was achieved with fluorescence detection at the end of separation microchannel, is given. Based on electropherograms, the number of theoretical separation plates is calculated (max. 70,000) and compared with data from the literature. Finally, some conclusions and observations on the design of the chip are given in order to improve its configuration in the future toward modular configuration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 261, 15 May 2018, Pages 474-480
Journal: Sensors and Actuators B: Chemical - Volume 261, 15 May 2018, Pages 474-480
نویسندگان
RafaÅ Walczak, Krzysztof Adamski, Wojciech Kubicki,