کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7141021 1462029 2018 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Highly sensitive electrochemiluminescence biosensor for cholesterol detection based on AgNPs-BSA-MnO2 nanosheets with superior biocompatibility and synergistic catalytic activity
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Highly sensitive electrochemiluminescence biosensor for cholesterol detection based on AgNPs-BSA-MnO2 nanosheets with superior biocompatibility and synergistic catalytic activity
چکیده انگلیسی
Monitoring the level of cholesterol in blood serum is a critical approach for diseases surveillance. In this work, we reported on the successful construction of a new electrochemiluminescence (ECL) sensor for cholesterol detection by employing the Ag-(bovine serum albumin) BSA-MnO2 nanosheets for cholesterol oxidase (ChOx) immobilization and catalytic amplification of ECL signal. The AgNPs-BSA-MnO2 nanosheets were synthesized by using BSA as protective agent, which greatly facilitated the immobilization of ChOx due to the existing amino group donated by BSA. The structure and properties of the AgNPs-BSA-MnO2 nanosheets were characterized by a series of techniques, including transmission electron microscope (TEM), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), powder X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR). Importantly, experiment results indicated that the synergistic effect of AgNPs with MnO2 nanosheets could provide excellent catalytic ability towards ROSs generation and thus to efficiently amplify the ECL signal of luminol-H2O2 system. Under optimal conditions, the ECL intensity increased with the increasing cholesterol concentration in a dynamic range from 0.21 μM to 1667 μM with a limit of detection (LOD) of 0.07 μM (3σ).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 260, 1 May 2018, Pages 642-649
نویسندگان
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