کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7141060 | 1462030 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Potentiometric enzyme biosensor for aflatoxin B1 detection - Kinetic simulation
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
We have investigated the operation of a potentiometric biosensor based on reversible acetylcholinesterase inhibition for determination of aflatoxin B1. The biochemical reactions between enzyme, substrate, and inhibitor are described using rate equations. A sensitivity study of the biosensor response to the biochemical reaction rates was done by varying each rate constant. The response to substrate and inhibitor was used as a fitting target for evaluation of the biochemical reaction rates. Initial concentrations of the enzyme (2.0â¯Ãâ¯10â5â¯M acetylcholinesterase), the substrate (4.0â¯Ãâ¯10â3â¯M acetylcholine chloride), and the inhibitor (9.6â¯Ãâ¯10â6â¯M aflatoxin B1) in the biosensor membrane act as the boundary conditions for the rate equations. This kinetic model allowed us to establish the effect of reaction rates on the biosensor response and reproduce the response to different inhibitor concentrations. The results may be used for biosensor design and optimization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 259, 15 April 2018, Pages 580-586
Journal: Sensors and Actuators B: Chemical - Volume 259, 15 April 2018, Pages 580-586
نویسندگان
Kateryna Stepurska, Sergei Dzyadevych, Sergii Gridin,