کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7143365 1462056 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Colorimetric detection of active botulinum neurotoxin using Cu2+ mediated gold nanoparticles agglomeration
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Colorimetric detection of active botulinum neurotoxin using Cu2+ mediated gold nanoparticles agglomeration
چکیده انگلیسی
We report a colorimetric detection of functional botulinum neurotoxin (BoNT) based on its proteolytic activity. Active BoNT can enzymatically cleave SNAptide, a short peptide with 15 amino acids. The cleaved peptides subsequently induced the agglomeration of gold nanoparticles, allowing a colorimetric signal that can be discerned by the naked eye at the low concentration of 1 ng/mL (6.67 pM), and achieve low limit of detection at 0.25 ng/mL (1.67 pM 3 σ/s) by spectral absorbance. This colorimetric detection will provide a simple and promising method for applications at low-resource sites.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 235, 1 November 2016, Pages 563-567
نویسندگان
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