کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7143484 | 1462055 | 2016 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Combined in vivo recording of neural signals and iontophoretic injection of pathway tracers using a hollow silicon microelectrode
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This paper presents the results of in vivo local release of a neuronal tracer, biotinylated dextran amine (BDA) in the rat somatosensory cortex using monolithically integrated microfluidic channel of a silicon neural microelectrode. The tracer injection is controlled by iontophoresis using Pt electrodes in the vicinity of the outlet of the microfluidic channel. Using 3-5 μA, 5-7 s on/off cycle and 15-20 min total injection time the localized injection resulted in clear anterograde and retrograde BDA labeling both within the cortex and in subcortical structures. Anterograde and retrograde labeling revealed the fine details of neuronal processes including dendritic spines and axon terminal-like endings. Injection sites appeared clear lacking any strong diffuse background labeling. Electrophysiological recording performed with the same microdevice immediately after the iontophoresis indicated normal cortical functioning. The results prove that the combination of in vivo multichannel neural recording and controlled tracer injection using a single implanted microdevice is feasible, and therefore it can be a powerful tool for studying the connectome of the brain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 236, 29 November 2016, Pages 815-824
Journal: Sensors and Actuators B: Chemical - Volume 236, 29 November 2016, Pages 815-824
نویسندگان
Z. Fekete, E. Pálfi, G. Márton, M. Handbauer, Zs. Bérces, I. Ulbert, A. Pongrácz, L. Négyessy,