کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7144305 | 1462062 | 2016 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effective improvement in performance of a miniature FIA-calorimetric biosensing system via denoising column addition and flow rate optimization
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The noise reduction and heat-response improvement of a miniature FIA-calorimetric biosensing system were evaluated for the determination of enzyme activity. An innovative denoising column was designed in combination with the use of a mathematical moving average for effectively decreasing signal noise from 30Â mV to 0.2Â mV. Additionally, various flow rates were tested for a comprehensive analysis of the influence of the flow rate on the heat response of this system. As the flow rate rose in the 0.1-0.5Â mL/min range, the temperature sensitivity of the calorimetric system increased linearly from 0.8Â mV/K to 1.184Â mV/K, showing an improvement of 48%. However, the exothermic peak integral reached the highest point at the flow rate of 0.3Â mL/min, when the system was used to detect the heat release of hydrolysis reaction catalysed by chicken liver esterase. Thus, the flow rate of 0.4Â mL/min was finally employed in this system. The biosensor was successfully applied for the detection of malathion, where 0.01Â mg/L malathion was detected significantly. Thus, this biosensor could potentially be used for the determinations of inhibitors based on enzyme inhibition methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 229, 28 June 2016, Pages 492-498
Journal: Sensors and Actuators B: Chemical - Volume 229, 28 June 2016, Pages 492-498
نویسندگان
Min Yuan, Jinsong Yu, Hui Cao, Fei Xu,