کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7144501 | 1462063 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sensitive detection of adenosine triphosphate by exonuclease III-assisted cyclic amplification coupled with surface plasmon resonance enhanced fluorescence based on nanopore
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Sensitive detection of adenosine triphosphate by exonuclease III-assisted cyclic amplification coupled with surface plasmon resonance enhanced fluorescence based on nanopore Sensitive detection of adenosine triphosphate by exonuclease III-assisted cyclic amplification coupled with surface plasmon resonance enhanced fluorescence based on nanopore](/preview/png/7144501.png)
چکیده انگلیسی
In traditional bioanalysis with mesoporous silica nanoparticle (MSN) as carrier, fluorescence quenching by encapsulation of fluorescence molecules in the pores was generally used. In the present work, Au nanoparticle@SiO2 mesoporous silica core-shell nanoparticles (AuNP@SiO2 MSN) was synthesized, and surface plasmon resonance enhanced fluorescence localized on nanopore was observed. In-hole fluorescence enhancement by MSN for detection of adenosine 5â²-triphosphate (ATP) was developed, on the base of exonuclease III (Exo III)-assisted cyclic amplification and surface plasmon resonance enhanced fluorescence localized on nanopore. Because of the autocatalytic target recycling amplification and the fluorescence enhancement, this designed protocol provided an ultrasensitive detection of ATP down to 0.1Â nM level, and can be utilized into cell lysates analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 228, 2 June 2016, Pages 509-514
Journal: Sensors and Actuators B: Chemical - Volume 228, 2 June 2016, Pages 509-514
نویسندگان
Xuemei Li, Yan Wang, Jie Luo, Shiyun Ai,