A Shear horizontal surface acoustic wave biosensor for a rapid and specific detection of d-serine

In this work, we report on the design and development of a surface acoustic wave (SAW) biosensor to detect the level of d-serine. The SAW-biosensor is based on the utilization of an inactivated form of the d-serine dehydratase isolated from Saccharomyces cerevisiae, a biomolecule that is able to specifically bind d-serine. In particular, we produced a PLP-reduced d-serine dehydratase biomolecule that was still able to bind d-serine and not to transform it into the product. The binding event between the “inactivated” d-serine dehydratase and d-serine was monitored by SAW delay-lines.