Study on rolling circle amplification of Ebola virus and fluorescence detection based on graphene oxide

A graphene oxide (GO) assisted rolling circle amplification (RCA) platform for Ebola virus (EBOV) detection is developed with simplicity and high sensitivity. In the absence of EBOV gene, no RCA products generated and the fluorescein amidate (FAM) labeled detection probe was adsorbed on the surface of GO, resulting in fluorescence quenching of the FAM. Addition of the EBOV gene allowed RCA to be taken place and the formation of double-stranded DNA (dsDNA) between RCA products and FAM labeled detection probe, leading to desorption of the FAM labeled detection probe from GO surface accompanied fluorescence recovery. EBOV gene can be determined both in aqueous solution and 1% serum solution. The limit of detection was 1.4 pM.