کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7144805 1462065 2016 27 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced cellular distribution and infiltration in a wet electrospun three-dimensional fibrous scaffold using eccentric rotation-based hydrodynamic conditions
ترجمه فارسی عنوان
توزیع و نفوذ سلولی پیشرفته در داربست فیبرهای سه بعدی الکترو اسپور با استفاده از شرایط هیدرودینامیکی مبتنی بر چرخش غیر عادی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Electrospinning technology is a versatile method for fabricating three-dimensional (3D) nanofibrous scaffolds using a wide range of polymeric materials for tissue engineering and regenerative medicine. However, a major concern regarding 3D electrospun scaffolds is that the densely packed layers hinder an even cellular distribution and in-depth infiltration. Here, we describe a new 'all-at-once' method enabling scaffold fabrication and cell seeding simultaneously, in which the medium bath containing cells is rotated eccentrically at high speed (>1500 rpm). The unstable flow of culture medium under hydrodynamic conditions resulted in a skein-shaped 3D structure and enhanced the even cellular distribution and in-depth infiltration. Cellular distribution and infiltration analyses confirmed that our method was superior to static and dynamic seeding methods. Moreover, we showed that our method facilitated long-term (14 days) proliferation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 226, April 2016, Pages 357-363
نویسندگان
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