A selective and sensitive fluorescent aptasensor for detection of kanamycin based on catalytic recycling activity of exonuclease III and gold nanoparticles

- Kanamycin is an aminoglycoside antibiotic, broadly used in human and veterinary for treatment of Gram-negative and Gram-positive infectious diseases.
- Overdosage of kanamycin could cause the serious side effects on human such as nephrotoxicity and ototoxicity.
- In this study, we developed a novel selective and sensitive fluorescent aptasensor for detection of kanamycin based on Exonuclease III and Gold nanoparticles with minimum consumption of aptamer for the first time.
- The designed aptasensor showed high selectivity toward aminoglycoside antibiotics, including kanamycin and gentamicin, with a limit of detection (LOD) as low as 437 pM for kanamycin.
- The designed aptasensor was successfully used to detect kanamycin in serum and milk.